RESUMEN
Objective: The objective of this study was to determine internal structure spectral profile of by-products from coffee processing that were affected by added-microorganism fermentation duration in relation to truly absorbed feed nutrient supply in ruminant system. Methods: The by-products from coffee processing were fermented using commercial fermentation product, consisting of various microorganisms: for 0 (control), 7, 14, 21, and 28 days. In this study, the association and correlation of carbohydrate-related spectral profiles with chemical and nutritional properties (chemical composition, total digestible nutrient, bioenergy values, carbohydrate sub-fractions and predicted degradation and digestion parameters as well as milk value of feed. The vibrational spectra of coffee by-products samples after fermentation for 0 (control), 7, 14, 21, and 28 days were determined using a JASCO FT/IR-4200 spectroscopy coupled with accessory of Attenuated total reflectance (ATR). The molecular spectral analyses with univariate approach were conducted with the OMNIC 7.3 software. Results: Molecular spectral analysis parameters in fermented and non-fermented by-products from coffee processing included Structural carbohydrate, Cellulosic compounds, Non-structural carbohydrates, Lignin compound, CH-bending, structural carbohydrate peak1, Structural carbohydrate peak2, Structural carbohydrate peak3, Hemicellulosic compound, Non-structural carbohydrate peak1, non-structural carbohydrate peak2, non-structural carbohydrate peak3. The study results show that added-microorganism fermentation induced chemical and nutritional changes of coffee by-products including carbohydrate chemical composition profiles, bioenergy value, feed milk value, carbohydrate subfractions, estimated degradable and undegradable fractions in the rumen, and intestinal digested nutrient supply in ruminant system. Conclusion: In conclusion, carbohydrate nutrition value changes by added-microorganism fermentation duration were in an agreement with the change of their spectral profile in the coffee by-products. The studies show that the vibrational ATR-FT/IR spectroscopic technique could be applied as a rapid analytical tool to evaluate fermented by-products and connect with truly digestible carbohydrate supply in ruminant system.
RESUMEN
Fast and simultaneous determination of inner quality parameters, such as fat and moisture contents, need to be predicted in cocoa products processing. This study aimed to employ the near-infrared reflectance spectroscopy (NIRS) in predicting the quality mentioned above parameters in intact cocoa beans. Near-infrared spectral data, in a wavelength ranging from 1000 to 2500 nm, were acquired for a total of 110 bulk cocoa bean samples. Actual fat and moisture contents were measured with standard laboratory procedures using the Soxhlet and Gravimetry methods, respectively. Two regression approaches, namely principal component regression (PCR) and partial least square regression (PLSR), were used to develop the prediction models. Furthermore, four different spectra correction methods, namely multiple scatter correction (MSC), de-trending (DT), standard normal variate (SNV), and orthogonal signal correction (OSC), were employed to enhance prediction accuracy and robustness. The results showed that PLSR was better than PCR for both quality parameters prediction. Spectra corrections improved prediction accuracy and robustness, while OSC was the best correction method for fat and moisture content prediction. The maximum correlation of determination (R2) and residual predictive deviation (RPD) index for fat content were 0.86 and 3.16, while for moisture content prediction, the R2 coefficient and RPD index were 0.92 and 3.43, respectively. Therefore, NIRS combined with proper spectra correction method can be used to rapidly and simultaneously predict inner quality parameters of intact cocoa beans.
